Rabbit Monoclonal Technology

Epitomics has developed a unique and proprietary method for making monoclonal antibodies from rabbits rather than the conventional method of starting with mice. The chart below demonstrates the general outline that Epitomics uses for producing a rabbit monoclonal antibody.

The basic principal for making the antibody is the same as for mouse monoclonals. Our proprietary Rabbit fusion partner cells can fuse to rabbit B-cells to create the Rabbit Hybridoma cells. Hybridomas are then screened to select for clones with specific and sensitive antigen recognition and the antibodies are characterized using a variety of methods.

Advantages of Rabbit Monoclonals

  • More diverse epitope recognition
  • Improved immune response to small-size epitopes
  • High specificity and affinity
  • Greatly improved response to mouse antigens

To put it simply - better antigen recognition. The rabbit immune system generates antibody diversity and optimizes affinity by mechanisms that are more efficient than those of mice and other rodents. This increases the possibility of obtaining a functional antibody that will work in a variety of applications.

Additionally, many small compounds and peptides do not elicit a good immune response in mice but do so in rabbits. It is often for this reason, that rabbit polyclonal antibodies are developed and used in many research and even some diagnostic applications, ranging from drug screening to clinical diagnosis. As an example of this, most of the phospho specific recognition antibodies being sold on the market today are rabbit polyclonals.

Patented Technology

Epitomics RabMab technology is the only currently available hybridoma based technology for making rabbit monoclonals and covered under the following U.S. Patents, No. 5,675,063 and 7,429,487. Our broad patents cover not only the rabbit fusion partner cell line but also the method for generating rabbit fusion partner cell line as well as the antibodies produced from the cell line.

Comparison of Rabbit and Mouse Monoclonal Antibodies

  Mouse Rabbit
Antigen recognition • Limited immuno response 
• Small molecules/peptides often non-immunogenic 
• Very limited response to rodent antigens
• Recognizes limited number of epitopes (due to immunodominance)
• High chance of success with range of antigens including small molecules and peptides • Good response to use rodent proteins
• Recognizes several epitopes per protein antigen

Affinities Nanomolar (~10-9 KD M) Picomolar (10-12 KD M) possible
Specificity Med-High High
Applications Westerns, ELISA, Flow Cytometry, IP, not always suitable for IHC, ICC. Westerns, ELISA, Flow Cytometry, IP, IHC, ICC (excellent results in IHC).

So what do these advantages mean to you?

With rabbit monoclonal antibodies you get the combined benefits of superior antigen recognition with the specificity and consistency of a monoclonal antibody, bringing you the highest possible quality antibody.

Wide range of applications possible - RabMab's have been shown to perform in a wide range of applications. Every one of our antibodies is screened and tested using ELISA, western blotting as well as ICC and IHC. Additionally many of our antibodies have been tested for use in Flow Cytometry. Antibodies available from most vendors are initially only tested for use in western blotting and so the use of the antibody in other applications is often not validated.

Check out the benefits of using Rabbit Monoclonal Antibodies for yourself

Rabbit Monoclonal antibody products  - Epitomics catalog antibodies

Custom Rabbit Monoclonal Antibodies - Take advantage of our patented tehnology

Comparison of Rabbit Monoclonals to Mouse Monoclonals - IHC comparison of Rabbit Monoclonals to leading commerically available mouse monoclonals.

We’ve made custom antibodies for a variety of pharmaceutical, biotech and academic researchers and would be happy to put our technology and expertise in antibody generation to work for you.