|
Epitomics has developed a unique
and proprietary method for making monoclonal antibodies from
rabbits rather than
the conventional method of starting with mice. Figure
1 demonstrates the general outline
that Epitomics uses for producing a rabbit monoclonal antibody.
The basic principal for making the antibody is the same as for
mouse monoclonals. Our proprietary
Rabbit fusion partner cells can fuse to rabbit B-cells to create
the Rabbit Hybridoma cells. Hybridomas
are then screened to select for clones with specific and sensitive
antigen recognition and the antibodies are characterized using
a variety of methods.
Advantages of Rabbit Monoclonals
• More diverse epitope recognition
• Improved immune response to small-size epitopes
• High specificity and affinity
• Greatly improved response to mouse antigens
To put it simply - better antigen recognition. The
rabbit immune system generates antibody diversity and optimizes affinity by
mechanisms that are more efficient than those of mice and other rodents. This increases
the possibility of obtaining a functional antibody that will work in a variety
of applications.
Additionally,
many small compounds and peptides do not elicit a good immune response
in mice but do so in rabbits. It is often for this reason, that
rabbit polyclonal antibodies are developed and used in many research
and even some diagnostic applications, ranging from drug screening
to clinical diagnosis. As an example
of this, most of the phospho specific recognition antibodies being
sold on the market today are rabbit polyclonals.
| |
Mouse |
Rabbit |
| Antigen recognition |
• Limited
immuno response
• Small molecules/peptides often non-immunogenic
• Very limited response
to rodent antigens
• Recognizes limited number
of epitopes (due to immunodominance) |
• High chance of
success with range of antigens including small molecules
and
peptides •
Good response to use rodent proteins
•
Recognizes several epitopes per protein antigen
|
| Affinities |
Nanomolar (~10-9 KD M) |
Picomolar (10-12 KD M)
possible |
| Specificity |
Med-High |
High |
| Applications |
Westerns, ELISA, Flow Cytometry, IP,
not always suitable for IHC, ICC. |
Westerns, ELISA, Flow
Cytometry, IP, IHC, ICC (excellent results in IHC). |
|
Table 1 – Comparison
of Rabbit
and Mouse Monoclonal Antibodies
So
what do these advantages mean to you ?
With rabbit monoclonal antibodies you get the combined benefits of
superior antigen recognition with the specificity and consistency
of a monoclonal antibody, bringing you the highest possible quality
antibody.
Wide range of applications possible – RabMab’s have been shown to
perform in a wide range of applications. Every
one of our antibodies is screened and tested using ELISA, western blotting as
well as ICC and IHC. Additionally many of our
antibodies have been tested for use in Flow
Cytometry. Antibodies available from most vendors
are initially only tested for use in western blotting and so the use of the antibody
in other applications is often not validated.
Check out the benefits of using Rabbit Monoclonal Antibodies
for yourself
Rabbit Monoclonal
antibody products
- Epitomics catalog antibodies
Custom Rabbit
Monoclonal Antibodies
- Take advantage of our patented tehnology
We’ve made custom antibodies for a variety of pharmaceutical,
biotech and academic researchers and would be happy to put our technology and
expertise in
antibody generation to work for you. |
