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  • High specificity and affinity
  • More diverse epitope recognition
  • Improved response to mouse antigens
  • Fully validated in multiple applications and species

What is the Rabbit Monoclonal Advantage?

Rabbit Monoclonal Antibodies (RabMAb®) provide the combined benefits of superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody, bringing you the highest quality antibody possible.

Published results from independent laboratories comparing rabbit monoclonal and mouse monoclonal antibodies have found RabMAbs to offer increased sensitivity with similar or better specificity.

Which one should you choose?

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Antigen
Recognition

Excellent response to a wide range of antigens Limited immuno response

Affinities

Picomolar (10-12 kD M) possible Nanomolar (~10-9 kD M)

Applications

Westerns, ELISA, Flow Cytometry, IP, IHC, ICC - Excellent results in IHC Westerns, ELISA, Flow Cytometry, IP Not suitable for mouse studies

CDX2


Paraffin-embedded human colonic carcinoma tissue stained with Epitomics' CDX2 RabMAb (Cat# 2475-1) and Vendor A's CDX2 Mouse Monoclonal (both at 1:1000 dilution factor)
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E-Cadherin


Paraffin-embedded human breast carcinoma tissue stained with Epitomics' E-Cadherin RabMAb (Cat# 1702-1, at 1:500 dilution factor) and Vendor A's E-Cadherin Mouse Monoclonal (at 1:50 dilution factor)
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HER2


Paraffin-embedded human breast carcinoma tissue stained with Epitomics' Her2 RabMAb and Vendor A's Her2 Mouse Monoclonal (both at 1:500 dilution factor)
HER-2Rabbit HER-2Mouse

Custom Antibody Services

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  • Having difficulties generating antibodies in mice?
  • Looking to develop phospho or other modification state antibodies?
  • Looking to develop antibodies against mouse proteins?

These are examples of situations in which we have helped customers develop high quality antibodies to move their research forward. Many of our customers have expressed that they have been able to generate a good rabbit polyclonal but have failed when trying to make the antibody in mice. The rabbit and mouse immune systems are very different and will thus respond to antigens differently. With our RabMAb® technology you can take advantage of the rabbits unique immune response and generate the monoclonal antibody you need for long term and consistent production.

Epitomics has the only true rabbit-rabbit hybridoma technology available and is the only technology that delivers customers with rabbit hybridoma cell lines.

Visit our webpage or give us a call to discuss your project today.

Rabbit Monoclonal Technology

Epitomics has developed a unique and proprietary method for making monoclonal antibodies from rabbits rather than the conventional method of starting with mice. The chart below demonstrates the general outline that Epitomics uses for producing a rabbit monoclonal antibody.


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The basic principal for making the antibody is the same as for mouse monoclonals. Our proprietary Rabbit fusion partner cells can fuse to rabbit B-cells to create the Rabbit Hybridoma cells. Hybridomas are then screened to select for clones with specific and sensitive antigen recognition and the antibodies are characterized using a variety of methods.

Rabbit vs. Mouse

Rabbit Monoclonal Antibodies (RabMAb®) provide the combined benefits of superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody, bringing you the highest quality antibody possible.

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Advantages of RabMAbs

  • More diverse epitope recognition
  • Improved immune response to small-size epitopes
  • High specificity and affinity
  • Greatly improved response to mouse antigens

To evaluate the specificity and sensitivity of Epitomics' RabMAbs, we compared various diagnostic marker specific RabMAbs with the leading commercially available Mouse Monoclonal counterparts. Both antibodies were tested on the same formalin fixed paraffin-embedded tissue sample and under their recommended immunohistochemistry conditions.

Results demonstrate that Epitomics' RabMAbs have higher sensitivity combined with lower background than the comparable mouse monoclonal antibodies.