Rabbit Polyclonal Ab Service
Our rabbit polyclonal service can be performed using either a peptide or protein immunogen. Please see below for an overview of our rabbit polyclonal service for peptide and protein immunizations.
Peptide Immunogen - Rabbit Polyclonal Service
For the peptide service customers may provide the conjugated peptide or we can perform the conjugation (Cat# CRP001). We can also offer peptide synthesis and cosultation on choosing the proper peptide sequence for an additional fee.
| Catalog # |
Peptide Conjugation |
|
| CRP001 |
Peptide conjugation - KLH (6-10 mg required)
Peptide conjugation - BSA (3-5 mg required) |
|
| CRP002 |
Peptide Immunization in 2 rabbits
|
Weeks |
| |
Pre-bleed
|
0 |
| Primary injection (0.5mg/ml - 1ml Complete Freund's Adjuvant) |
1 |
| 1st Boost (0.25mg/ml - 1ml Incomplete Freund's Adjuvant) |
3 |
| 2nd Boost (0.25mg/ml - 1ml IFA) |
5 |
| 3rd Boost (0.25mg/ml - 1ml IFA) |
7 |
| Bleed #1 (25ml serum) |
8.5 |
| Bleed #2 (25ml serum) |
9 |
| 4th Boost (0.25mg/ml - 1ml IFA) |
9.5 |
| Bleed #3 (25ml serum) |
11 |
| Bleed #4 (25ml serum) |
11.5 |
| Titration- Elisa |
|
| CRP003 |
Project: Ab purification |
|
| |
Purification- affinity purified - mg |
|
| CRP004 |
Project: Additional testing |
|
| |
WB, IHC, IP |
|
Protein Immunogen - Rabbit Polyclonal Service
For the protein service customers supply the antigen of their choosing. Amount of protein ideally required is 5 - 6mg.
| Catalog # |
Project: Protein |
Weeks |
| CRP006 |
Protein Immunization in 2 rabbits
|
|
| |
Pre-bleed
|
0 |
| Primary injection (0.4mg/ml - 1ml Complete Freund's Adjuvant) |
1 (day) |
| 1st Boost (0.2mg/ml - 1ml Incomplete Freund's Adjuvant) |
3 |
| 2nd Boost (0.25mg/ml - 1ml IFA) |
5 |
| Bleed #1 (25ml serum) |
6.5 |
| Bleed #2 (25ml serum) |
7 |
| 3rd Boost (0.2mg/ml - 1ml IFA) |
7.5 |
| Bleed #3 (25ml serum) |
9 |
| Bleed #4 (25ml serum) |
9.5 |
| Titration- Elisa |
|
| CRP007 |
pAb purification |
|
| |
Purification- affinity purified - mg |
|
| CRP008 |
Additional testing |
|
| |
WB, IHC, IP |
|
Submit a custom Ab enquiry on-line
You can also contact
us directly via telephone at 650.583.6688
or via email at info@epitomics.com |
