Rabbit monoclonal antibodies for neutralization and activation/proliferation
Epitomics' RabMab technology allows for the development of monoclonal antibodies with superior antigen recognition and versatility for use in various demanding applications. Utilizing these unique benefits, Epitomics has developed high quality rabbit monoclonals for neutralization and/or activation for biological activity studies to various protein targets.
Neutralizing a specific target of protein with Bioactive antibodies can give valuable insight into a protein's biological activities. Additionally, neutralization can be utilized to assist in measuring the biological activity of samples containing several bioactive proteins.
|Blocking ligand-receptor interaction – Approximately 0.6 ug/ml of this antibody blocks 50% of the binding of 0.5 ug/ml of hVEGF165 to immobilized rhKDR (50 μl of a 5 μg/ml solution was coated on a 96-well plate) in an ELISA assay.||Inhibition of HUVEC cell proliferation by anti-human VEGF antibody (cat# 4408-1) -To measure the ability of the antibody to neutralize the bioactivity of hVEGF on HUVEC cells, 0.4 ng/ml of rhVEGF was incubated with various concentrations of the antibody for 1.5 hour at 37°C in a 96-well plate before 1 x104 cells of HUVEC cells were added per well. The assay mixture in a total volume of 200 μl, containing antibody at the concentrations indicated, was incubated at 37°C for 72 hours. Then 20 ul alamar blue was added and incubated for a suitable time at 37°C. The plate was read using a fluorescence system (excitation at 530 nm and emission at 590 nm). The IC50 of this lot of antibody was approximately 4-10 ng/ml.|
For activation/proliferation, bioactive antibodies can be used to induce biological activities in samples by acting as the inducing agents.