p70 S6 Kinase (RPS6KB1) antibody 
RabMAb®
Cat.#: 1175-1
Rabbit Monoclonal Antibody
Swiss Prot: P23443
Mol Weight: 70kDa
Price: $260
Availability: Ship next business day
Description
p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2).
Recommended Applications
WB, IHC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000-1:10,000
IHC: 1:50
IP: 1:60
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on 293T cell lysate using anti-p70 S6 Kinase RabMAb (cat. #1175-1). | B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-p70 S6 Kinase RabMAb (cat. #1175-1). |
Specificity
A synthetic peptide corresponding to residues human p70 S6 kinase was used as an immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RPS6KB1 antibody, PS6K antibody, S6K antibody, S6K1 antibody, STK14A antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, Serine/threonine-protein kinase 14A antibody, p70 ribosomal S6 kinase alpha antibody
Description References
1. Pearson, R.B. and G. Thomas. Regulation of p70s6k/p85s6k and its role in the cell cycle. Prog Cell Cycle Res. 1: 21
2. Pullen, N. and G. Thomas. The modular phosphorylation and activation of p70s6k. FEBS Lett. 410: 78
Product References
1. Birle DC and Hedley DW, Signaling interactions of rapamycin combined with erlotinib in cervical carcinoma xenografts ,Mol Cancer Ther 5 (2006) 2494–2502
[Application: WB]
2. Zhang YJ, Zhao SL, Tian XQ, et al, Combined inhibition of Dnmt and mTOR signaling inhibits formation and growth of colorectal cancer ,Int J Colorectal Dis 24 (2009) 629-639
[Application: IHC]
3. Zhang YJ, Dai Q, Sun DF, et al, mTOR Signaling Pathway Is a Target for the Treatment of Colorectal Cancer ,Ann Surg Oncol 16 (2009) 2617-2628
[Application: IHC]
4. Xiao L, Wang YC, Li WS, et al, The role of mTOR and phospho-p70S6K in pathogenesis and progression of gastric carcinomas: an immunohistochemical study on tissue microarray ,J Exp Clin Cancer Res 28 (2009) : 152
[Application: IHC]
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