p70 S6 Kinase Phospho (pT421/pS424) (RPS6KB1) antibody 
RabMAb®
Cat.#: 1135-1
Rabbit Monoclonal Antibody
Swiss Prot: P23443
Mol Weight: 70kDa
Price: $285
Availability: Ship next business day
Description
p70 S6 Kinase is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1). p70 S6 kinase phosphorylates specifically ribosomal protein S6. Activation of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (2). Activation occurs via phosphorylation at Ser411, Thr421 and Ser424 within the pseudosubstrate region (3, 4).
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:2,500
IP: 1:20
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on 293T cell lysate using anti-Phospho-p70 S6 Kinase (pT421/pS424) RabMAb (cat. #1135-1); dilution 1:2,500. Cell lysates were either (1) untreated or (2) treated with EGF. |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Thr421 and Ser424 of human p70 S6 Kinase alpha was used as immunogen. The antibody only detects p70-S6K phosphorylated on Threonine 421 and Serine 424.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RPS6KB1 antibody, PS6K antibody, S6K antibody, S6K1 antibody, STK14A antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, Serine/threonine-protein kinase 14A antibody, p70 ribosomal S6 kinase alpha antibody
Description References
1. Pearson, R.B. and G. Thomas. Regulation of p70s6k/p85s6k and its role in the cell cycle. Prog Cell Cycle Res. 1: 21
2. Pullen, N. and G. Thomas. The modular phosphorylation and activation of p70s6k. FEBS Lett. 410: 78
3. Dufner, A. and G. Thomas. Ribosomal S6 kinase signaling and the control of translation. Exp Cell Res. 253: 100
4. Le, X.F., et al. Paclitaxel induces inactivation of p70 S6 kinase and phosphorylation of Thr421 and Ser424 via multiple signaling pathways in mitosis. Oncogene 22: 484
Product References
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