c-Myc (N-term) (MYC) antibody 
RabMAb®
Cat.#: 1472-1
Rabbit Monoclonal Antibody
Swiss Prot: P01106
Mol Weight: 57kDa
Price: $260
Availability: Ship next business day
Description
Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:250-500
IP: 1:150
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |  |
| A. Western blot analysis of Jurkat cell lysate using anti-c-Myc (N-term) RabMAb (catalog #1472-1), dilution 1:10,000. | B. Immunohistochemical analysis of paraffin-embedded human skin carcinoma using anti-c-Myc (N-term) RabMAb (catalog #1472-1). | C. Immunofluorescent staining of HeLa cells using anti-c-Myc (N-term) RabMAb (catalog #1472-1). |
Specificity
A synthetic peptide corresponding to residues in N-terminus of human c-Myc was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
MYC antibody, MRTL antibody, bHLHe39 antibody, c-Myc antibody, Myc proto-oncogene protein antibody, Proto-oncogene c-Myc antibody, Transcription factor p64 antibody
Description References
1. Nisen, P.D. et al. Enhanced expression of the N-myc gene in Wilms
2. Nau, M.N. et al. L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer. Nature 318: 69
3. Gupta, S. et al. Transactivation of Gene Expression by Myc is Inhibited by Mutation at the Phosphorylation Sites Thr-58 and Ser-62. Proc. Natl. Acad. Sci. USA 90: 3216
Product References
1. Nakles RE, Shiffert MT, Díaz-Cruz ES, et al, Altered AIB1 or AIB1 deta 3 Expression Impacts ERα Effects on Mammary Gland Stromal and Epithelial Content ,Mol Endocrinol 25 (2011) 549-563
2. Zeng HW, Wu M, Botnen JH, Methylselenol, a Selenium Metabolite, Induces Cell Cycle Arrest in G1 Phase and Apoptosis via the Extracellular-Regulated Kinase 1/2 Pathway and Other Cancer Signaling Genes ,J. Nutr.139 (2009) 1613 - 1618
3. Rapisarda A and Melillo G, UVC inhibits HIF-1a protein translation by a DNA damage- and topoisomerase I-independent pathway ,Oncogene 26 (2007) 6875–6884
[Application: WB]
4. Huang TH, Wu FT, Loeb GB, et al, Up-regulation of miR-21 by HER2/neu Signaling Promotes Cell Invasion ,J. Biol. Chem. 284 (2009) 18515 - 18524
[Application: WB]
5. Zeng HW, Wu M, Botnen JH, Methylselenol, a Selenium Metabolite, Induces Cell Cycle Arrest in G1 Phase and Apoptosis via the Extracellular-Regulated Kinase 1/2 Pathway and Other Cancer Signaling Genes ,J. Nutr. 139 (2009) 1613 - 1618
[Application: WB]
6. Lee HG, Chen Q, Wolfram JA, et al, Cell Cycle Re-Entry and Mitochondrial Defects in Myc- Mediated Hypertrophic Cardiomyopathy and Heart Failure ,PLoS One 4 (2009) e7172
[Application: ICC]
7. Gurel B, Iwata T, Koh CM, et al, Nuclear MYC protein overexpression is an early alteration in human prostate carcinogenesis ,Mod Pathol 21 (2008) 1156-1167
[Application: WB, IHC]
8. Zeng H, Wu M, Botnen JH, Methylselenol, a selenium metabolite, induces cell cycle arrest in G1 phase and apoptosis via the extracellular-regulated kinase 1/2 pathway and other cancer signaling genes ,J Nutr 139 (2009) 1613-1618
[Application: WB]
9. Melton C, Judson RL, Blelloch R, Opposing microRNA families regulate selfrenewal in mouse embryonic stem cells ,Nature 463 (2010) 621-626
[Application: WB]
10. Wolfer A, Wittner BS, Irimia D, et al, MYC regulation of a “poor-prognosis” metastatic cancer cell state ,Proc Natl Acad Sci U S A 107 (2010) 3698-3703
[Application: IHC]
11. Iwata T, Schultz D, Hicks J, et al, MYC Overexpression Induces Prostatic Intraepithelial Neoplasia and Loss of Nkx3.1 in Mouse Luminal Epithelial Cells ,PLoS One 5 (2010) e9427
[Application: WB]
12. Chen S, Blank JL, Peters T, et al, Genome-Wide siRNA Screen for Modulators of Cell Death Induced by Proteasome Inhibitor Bortezomib ,Cancer Res 70 (2010) 4318-4326
13. Ruzinova MB, Caron T, Rodig SJ, Altered Subcellular Localization of c-Myc Protein Identifies Aggressive B-cell Lymphomas Harboring c-MYC Translocation ,Am J Surg Pathol 34 (2010) 882-891
[Application: IHC]
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