ZAP-70 (ZAP70) antibody 
RabMAb®
Cat.#: 1491-1
Rabbit Monoclonal Antibody
Swiss Prot: P43403
Mol Weight: 70kDa
Price: $260
Availability: Ship next business day
Description
Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1000-10,000
IHC: 1:100-250
ICC: 1:100-250
IP: 1:50
FC: 1:80
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on Jurkat cell lysate using anti-ZAP-70 RabMAb (cat. #1491-1); dilution 1:500. | B. Immunohistochemical analysis of paraffin-embedded human lynph node using anti-ZAP-70 RabMAb (catalog #1491-1). |
Specificity
A synthetic peptide corresponding to residues in C-terminus of human ZAP-70 was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ZAP70 antibody, FLJ17670 antibody, FLJ17679 antibody, SRK antibody, STD antibody, TZK antibody, ZAP-70 antibody, Tyrosine-protein kinase ZAP-70 antibody, 70 kDa zeta-associated protein antibody, Syk-related tyrosine kinase antibody
Description References
1. Chu, D.H., et al. The Syk family of protein tyrosine kinases in T-cell activation and development. Immunol. Rev. 165: 167
2. Isakov, N., et al. ZAP-70 binding specificity to T cell receptor tyrosine-based activation motifs: the tandem SH2 domains of ZAP-70 bind distinct tyrosine-based activation motifs with varying affinity. J Exp Med. 181: 375
3. Di Bartolo, V., et al. Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. J Biol Chem. 274: 6285
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