Src (SRC) antibody 
RabMAb®
Cat.#: 2778-1
Rabbit Monoclonal Antibody
Swiss Prot: P12931
Mol Weight: 60kDa
Price: $260
Availability: Ship next business day
Description
Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).
Recommended Applications
WB, IHC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |
| Western blot analysis on A) U87-MG and B) SH-5YSY cell lysates using Rabbit anti-Src (cat# 2778-1). |
Specificity
A synthetic peptide corresponding to residues to human Src was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
SRC antibody, ASV antibody, SRC1 antibody, c-SRC antibody, p60-Src antibody, Proto-oncogene tyrosine-protein kinase Src antibody, Proto-oncogene c-Src antibody, pp60c-src antibody
Description References
1. Cooper, J. A., and King, C. S. (1986) Mol. Cell. Biol. 6, 4467-4477 2. Courtneidge, S. A. (1985) EMBO J. 4, 1471-1477 3. Okada, M., Howell, B. W., Broome, M. A., and Cooper, J. A. (1993) J. Biol. Chem. 268, 18070-18075 4. Leiming Li, Masaya Okura, Akira Imamoto Molecular and Cellular Biology, Feb. 2002, p. 1203-1217
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