RSK1 (N-term) (RPS6KA1) antibody 
RabMAb®
Cat.#: 2004-1
Rabbit Monoclonal Antibody
Swiss Prot: Q15418
Mol Weight: 83kDa
Price: $260
Availability: Ship next business day
Description
RSK 1 (p90-RSK 1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes RSK 1, RSK 2 and RSK 3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-RSK 1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1000
IHC: 1:100
ICC: 1:200
IP: 1:10
FC: 1:50
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on MCF7 cell lysate using anti-Rsk1 (N-term) RabMAb (catalog #2004-1), dilution 1:1000. | B. IHC - Colon tissue section stained for RSK-1 subcellular location using anti-RSK-1. | C. Flow cytometric analysis of permeabilized Jurkat cells using anti-Rsk-1 RabMAb (red) (catalog #2004-1) or a rabbit IgG (negative) (green). |
 | ||
| D. Immunofluorescent staining of HeLa cells using anti-Rsk 1 RabMab (cat#1004-1). |
Specificity
A synthetic peptide corresponding to residues in N-terminus of human p90-RSK1 was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RPS6KA1 antibody, HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Ribosomal protein S6 kinase alpha-1 antibody, Ribosomal S6 kinase 1 antibody
Description References
1. Dalby, K.N. et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273, 1496-1505 (1998)
2. Frodin, M. and Gammeltoft, S. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151, 65
3. Lazar, D. F. et al. Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin. J. Biol. Chem. 270, 20801
4. Chen, R. et al. Phosphorylation of the c-Fos Transrepression Domain by Mitogen-Activated Protein Kinase and 90-kDa Ribosomal S6 Kinase. Proc. Natl. Acad. Sci. USA, 90, 10952
Product References
1. Shrum CK, DeFrancisco D, Meffert MK, Stimulated nuclear translocation of NF-κB and shuttling differentially depend on dynein and the dynactin complex ,PNAS 106 (2009) 2647 - 2652
[Application: WB]
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