RSK1 Phospho (pS380) (RPS6KA1) antibody 
RabMAb®
Cat.#: 1468-1
Rabbit Monoclonal Antibody
Swiss Prot: Q15418
Mol Weight: 90kDa
Price: $285
Availability: Ship next business day
Description
RSK 1 (p90-RSK 1) is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes RSK 1, RSK 2 and RSK 3. These are broadly expressed serine/threonine protein kinases (1) activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2 (2). p90-RSK 1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB (2,4).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:250-500
IP: 1:80
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis of A431 using anti-RSK-1 (pS380) RabMAb (catalog #1468-1), dilution 1:10,000. A431 cells were either untreated (A) or treated with EGF (B) | B. Immunofluorescent staining of A431 cells using anti-RSK-1 (pS380) RabMAb (catalog #1468-1). |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser380 of human p90RSK1 was used as immunogen. The antibody only detects RSK1 phosphorylated on Serine 380.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
RPS6KA1 antibody, HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Ribosomal protein S6 kinase alpha-1 antibody, Ribosomal S6 kinase 1 antibody
Description References
1. Dalby, K.N. et al. Identification of Regulatory Phosphorylation Sites in Mitogen-activated Protein Kinase (MAPK)-activated Protein Kinase-1a/p90rsk That Are Inducible by MAPK. J. Biol. Chem. 273, 1496-1505 (1998).
2. Frodin, M. and Gammeltoft, S. Role and regulation of 90 kDa ribosomal S6 kinase (RSK) in signal transduction. Mol. Cell. Endocrinol. 151, 65
3. Lazar, D. F. et al. Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin. J. Biol. Chem. 270, 20801
4. Chen, R. et al. Phosphorylation of the c-Fos Transrepression Domain by Mitogen-Activated Protein Kinase and 90-kDa Ribosomal S6 Kinase. Proc. Natl. Acad. Sci. USA, 90, 10952
Product References
1. Chaturvedi D, Gao X, Cohen MS, et al, Rapamycin induces transactivation of the EGFR and increases cell survival ,Oncogene 28 (2009) 1187–1196
[Application: WB]
2. Chaturvedi D, Cohen MS, Taunton J, et al, The PKARIα Subunit of Protein Kinase A Modulates the Activation of p90RSK1 and Its Function ,J. Biol. Chem. 284 (2009) 23670 - 23681
[Application: WB]
3. Gao X, Patel TB, Regulation of Protein Kinase A Activity by p90 Ribosomal S6 Kinase 1 ,J Biol Chem 284 (2009) 33070-33078
[Application: WB]
4. Gao X, Chaturvedi D, Patel TB, p90 Ribosomal S6 Kinase 1 (RSK1) and the Catalytic Subunit of Protein Kinase A (PKA) Compete for Binding the Pseudosubstrate Region of PKAR1 α: role in the regulation of PKA and RSK1 activities ,J Biol Chem 285 (2010) 6970-6979
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