MSH6 antibody RabMAb®

Cat.#: 2846-1
Abcam ID: ab92471

Rabbit Monoclonal Antibody

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Description

The MutS homologue 6 protein (MSH6) is a member of the MutS homolog family required in the DNA mismatch repair system (1). MSH6 forms a MutS alpha dimer with MSH2, binding to DNA mismatches to initiate DNA repair (2). MutS alpha bends the DNA helix and recognizes single base mismatches and dinucleotide insertion-deletion loops in the DNA (2). Heterozygous mutations in the MSH6 gene are a cause of hereditary nonpolyposis colorectal cancer (HNPCC), forming a specific mispair binding complex with MSH3 (3, 4). The frequency of MSH6 mutation is higher in HNPCC than in atypical HNPCC (5). The MSH2/MSH6 dimer may also play a role in DNA homologous recombination repair (2).

Recommended Applications

WB, IHC, IP

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IHC: 1: 100 - 250
IP: 1: 10 - 100

Species Reactivity *

Human, Mouse

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

Western blot analysis on (A) A431, (B) HeLa, and (C) SW480 cell lysates using anti-MSH6 RabMAb (cat. # 2846-1).	Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using anti-MSH6 RabMAb (cat. #2846-1).

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Specificity

A synthetic peptide corresponding to residues at the N-terminus in human MSH6 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

MSH6 antibody, GTBP antibody, HNPCC5 antibody, HSAP antibody, DNA mismatch repair protein Msh6 antibody, MutS-alpha 160 kDa subunit antibody

Description References

1. Online Mendelian Inheritance in Man, OMIM (TM). Johns Hopkins University, Baltimore, MD. MIM Number: {600678}: {4/7/2009}: . World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/ 2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009) 3. Guerrette et al. Mol Cell Biol. 1998 Nov;18(11):6616-23. 4. Wimmer et al. Hum Genet. 2008 Sep;124(2):105-22. 5. Zhao et al. J Toxicol Environ Health A. 72(11):690-7, 2009.

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