Ki-67 (MKI67) antibody RabMAb®

Cat.#: 2746-1
Abcam ID: ab92742

Rabbit Monoclonal Antibody

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Description

Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in nuclear matrix in the later phases (1). Ki-67 is thought to be involved in the maintaining of cell proliferation, however, its exact mechanism for function is unknown. Due to the fact that Ki-67 can be used to determine the growth fraction (the proportion of the cells committed to the cell cycle), it is consider an important marker in human tumor diagnostics and prognostics (2).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:500 - 1,000
IHC: 1:500 - 1,000
ICC: 1:250 - 500

Species Reactivity

Human

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Products Data

Western blot analysis on HeLa lysates using anti-Ki-67 RabMAb (cat. #2746-1).	Immunohistochemical analysis of paraffin-embedded human tonsil using anti-Ki-67 RabMAb (cat. #2746-1).	Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma using anti-Ki-67 RabMAb (cat. #2746-1).
Immunofluorescent staining of HeLa cells using anti-Ki-67 RabMAb (cat. #2746-1).

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Specificity

A synthetic peptide corresponding to residues in human Ki-67 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

MKI67 antibody, KIA antibody, Antigen KI-67 antibody

Description References

1. Gerdes J Semin Cancer Biol 1(3):199-206, 1990. 2. Gerdes J, Scholzen T J Cell Physiol 182(3):311-322, 2000.

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