Histone H3 Phospho (pS10) DyLight® 488 (HIST3H3) antibody RabMAb®Cat.#: 4521-1
Abcam ID: ab139848
Rabbit Monoclonal Antibody
Swiss Prot: Q16695
Mol Weight: 17kDa
Price: Contact Abcam
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5).
Applications and Recommended Dilution Factors
ICC: 1:50 - 100
Species Reactivity *
*DyLight is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
|Immunofluorescent analysis of Hela cells using Histone H3 pS10 RabMAb (DyLight 488 conjugate).|
A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.
Storage Condition and Buffer
Supplied in PBS (pH 7.2), 0.02% sodium azide, 0.05% BSA. Store at 4℃. Protect from light. Stable for 6 months from date of receipt.
HIST3H3 antibody, H3.4 antibody, H3/g antibody, H3FT antibody, H3t antibody, Histone H3.1t antibody, H3/g antibody
1. Braunstein, M., et al. Mol. Cell. Biol. 16: 4349–56 (1996). 2. Workman, J.L. and R.E. Kingston. Annu. Rev. Biochem. 67: 545–579 (1998). 3. Hansen, J.C. et al. Biochemistry 37, 17637–17641 (1998). 4. Strahl, B.D. and C.D. Allis. Nature 403, 41–45 (2000). 5. Hendzel, M.J., et al. Chromosome 106: 348–360 (1997).
|Product Name||Catalog #|
|Histone H3 Acetyl (K9) RabMAb®||1328-1|
|Histone H3 Dimethyl (K4) RabMAb®||1347-1|
|Histone H3 Dimethyl (K9) RabMAb®||1349-1|
|Histone H3 Phospho (pS10) RabMAb®||1173-1|
|Histone H3 Phospho (pS28) RabMAb®||1329-1|
|Histone H3 Acetyl (K9) Alexa Fluor® 488 RabMAb®||4629-1|
|Histone H3 Phospho (pS10) Alexa Fluor® 488 RabMAb®||4631-1|