HER2 / ErbB2 (ERBB2) antibody RabMAb®

Cat.#: 2064-1
Abcam ID: ab134182

Rabbit Monoclonal Antibody

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Description

HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.

Recommended Applications

WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:250 - 500
IP: 1:50
FC: 1:30

Species Reactivity *

Human

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on SKBR-3 cell lysate using anti-HER2 / ErbB2 RabMAb (cat. # 2064-1).	B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-HER2 / ErbB2 RabMAb (cat. #2064-1).	Immunofluorescent staining of SKBR cells using anti-HER2 / ErbB2 RabMAb (cat. #2064-1).

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Specificity

A synthetic peptide corresponding to residues near the C-terminus of human Her2 was used as an immunogen. This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorylated Her2.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ERBB2 antibody, CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Receptor tyrosine-protein kinase erbB-2 antibody, Proto-oncogene Neu antibody, Proto-oncogene c-ErbB-2 antibody, Tyrosine kinase-type cell surface receptor HER2 antibody, p185erbB2 antibody

Description References

1. Muthuswamy, S. K., et al. Mol. Cell. Biol. 19: 6845-6857 (1999).
2. Qian, X., et al. Proc. Natl. Acad. Sci. USA 91: 1500
3. Dittadi, R, et al. J. Natl. Cancer Inst. 92: 1443-1444 (2000).
4. Klapper, L. N., et al. Cancer Res. 60: 3384-3388 (2000).

Product References

1. Kawano S, Ikeda W, Kishimoto M, et al, Silencing of ErbB3/ErbB2 Signaling by Immunoglobulin-like Necl-2 ,J. Biol. Chem. 284 (2009) 23793 - 23805
[Application: IP]

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