GRB2 (C-term) antibody 
RabMAb®
Cat.#: 1517-1
Rabbit Monoclonal Antibody
Swiss Prot: P62993
Mol Weight: 25kDa
Price: $260
Availability: Ship next business day
Description
The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:100-250
ICC: 1:50-100
IP: 1:50
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on 293 cell lysate using anti-GRB2 (C-term) RabMAb (catalog #1517-1), dilution 1:10,000. | B. Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti- GRB2 (C-term) RabMAb (catalog #1517-1). | C. Immunofluorescent staining of HeLa cells using anti-GRB2 (C-term) RabMAb (catalog #1517-1). |
Specificity
A synthetic peptide corresponding to residues in the C-terminus of human GRB2 was used as an immunogen. This antibody can also detect the splice isoform GRB3-3 of GRB2.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
GRB2 antibody, ASH antibody, EGFRBP-GRB2 antibody, Grb3-3 antibody, MST084 antibody, MSTP084 antibody, NCKAP2 antibody, Growth factor receptor-bound protein 2 antibody, Adapter protein GRB2 antibody, Protein Ash antibody, SH2/SH3 adapter GRB2 antibody
Description References
1. Matuoka, K.; Shibata, M.; Yamakawa, A.; Takenawa, T.: Proc. Nat. Acad. Sci. 89: 9015-9019, 1992
2. Lowenstein, E. J.; Daly, R. J.; Batzer, A. G.; Li, W.; Margolis, B.; Lammers, R.; Ullrich, A.; Skolnik, E. Y.; Bar-Sagi, D.; Schlessinger, J.: Cell 70: 431-442, 1992.
3. Nioche P.; et al, JMB, Volume 315, Number 5, February ( 2002), 1167-1177(11)
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