Filamin A (C-term) (FLNA) antibody 
RabMAb®
Cat.#: 2242-1
Rabbit Monoclonal Antibody
Swiss Prot: P21333
Mol Weight: 280kDa
Price: $260
Availability: Ship next business day
Description
Filamins are a family of high molecular mass cytoskeletal proteins that organize filamentous actin in networks and stress fibers. Recently it has become clear that filamins anchor various transmembrane proteins to the actin cytoskeleton and provide a scaffold for a wide range of cytoplasmic signaling proteins (1). Actin-binding protein (Filamin A) is a ubiquitous dimeric actin cross-linking phosphoprotein of peripheral cytoplasm, where it promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins (2). The actin-binding domain is near the amino-terminus of the subunit where the amino acid sequence is similar to other actin filament binding proteins, including alpha-actinin, beta-spectrin, dystrophin, and Dictyostelium abp-120. A monoclonal antibody recognizing Filamin A and Fh1 binds to blood vessels, astrocytes, neurofibrillary tangles, neuropil threads, and dystrophic neurites in the AD brain. Detection of Filamin A /Fh1 proteins in these structures suggests that these presenilin-interacting proteins may be involved in the development of Alzheimer's disease and that interactions between presenilins and Filamin A /Fh1 may be functionally significant (3).
Recommended Applications
WB, IHC, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
FC: 1:40
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A. Western blot analysis on (A) COS-1 (B) HeLa (C) 3T3 and (D) C6 cell lysate using anti-Filamin A RabMAb (cat. # 2242-1), dilution 1:500,000. | B. Immunohistochemical analysis of paraffin-embedded human uterus using anti-Filamin A RabMAb. (cat. #2242-1) | C. Immunofluorescent staining of HeLa cells using anti-Filamin A RabMAb. (cat. #2242-1). |
Specificity
A synthetic peptide corresponding to residues near the C-terminus of human Filamin A was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
FLNA antibody, ABP-280 antibody, ABPX antibody, CVD1 antibody, DKFZp434P031 antibody, FLN antibody, FLN-A antibody, FLN1 antibody, FMD antibody, MNS antibody, NHBP antibody, OPD antibody, OPD1 antibody, OPD2 antibody, XLVD antibody, XMVD antibody, Filamin-A antibody, Alpha-filamin antibody, Endothelial actin-binding protein antibody, Filamin-1 antibody, Non-muscle filamin antibody
Description References
1. van der Flier A. Biochim Biophys 1538(2):99-117, 2001
2. Gorlin JB, et al. J Cell Biol 111(3):1089-105, 1990
3. Zhang W, et al. J Neurosci 18(3):914-22, 1998.
Product References
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