Cbl Phospho (pY700) (CBL) antibody 
RabMAb®
Cat.#: 2375-1
Rabbit Monoclonal Antibody
Swiss Prot: P22681
Mol Weight: 120kDa
Price: $285
Availability: Ship next business day
Description
c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). It has been demonstrated that tyrosines 700, 731, and 774 are the major tyrosine phosphorylation sites of c-Cbl in T cells in response to pervanadate treatment, as well as in response to TcR/CD3 ligation (5).
Recommended Applications
WB, IP, FC
Applications and Recommended Dilution Factors
WB: 1:5000 - 10,000
IP: 1:40
FC: 1:50
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on Jurkat cell lysate using anti-Phospho-Cbl (pY700) RabMAb (cat. #2375-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with pervanadate. |
Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 700 were used as an immunogen. This antibody detects Cbl phosphorylated on tyrosine 700.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CBL antibody, C-CBL antibody, CBL2 antibody, RNF55 antibody, E3 ubiquitin-protein ligase CBL antibody, Casitas B-lineage lymphoma proto-oncogene antibody, Proto-oncogene c-Cbl antibody, RING finger protein 55 antibody, Signal transduction protein CBL antibody
Description References
1. Langdon, W. Y., et al. Proc. Natl. Acad. Sci. USA 86: 1168-72 (1989).
2. Blake, T. J., et al. EMBO J. 12: 2017-26 (1993).
3. Joazeiro, C. A., et al. Science 286: 309-12 (1999).
4. Feshchenko EA, et al. J Biol Chem, 272(14):8323-31, 1998
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