Cbl (C-term) (CBL) antibody 
RabMAb®
Cat.#: 1486-1
Rabbit Monoclonal Antibody
Swiss Prot: P22681
Mol Weight: 120kDa
Price: $260
Availability: Ship next business day
Description
c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4).
Recommended Applications
WB, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100
IP: 1:100
FC: 1:100
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on Jurkat cell lysates using anti-Cbl (C-term) RabMAb (catalog #1486-1), 1:5,000 dilution. | B. Immunofluorescent staining of HeLa cells using anti-Cbl (C-term) RabMAb (catalog #1486-1). |
Specificity
A synthetic peptide corresponding to residues in C-terminus of human Cbl was used as immunogen. Predicted to cross-react with chicken, based on sequence homology.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CBL antibody, C-CBL antibody, CBL2 antibody, RNF55 antibody, E3 ubiquitin-protein ligase CBL antibody, Casitas B-lineage lymphoma proto-oncogene antibody, Proto-oncogene c-Cbl antibody, RING finger protein 55 antibody, Signal transduction protein CBL antibody
Description References
1. Langdon, W. Y., et al. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas. Proc. Natl. Acad. Sci. USA 86: 1168
2. Blake, T. J., et al. The truncation that generated the v-cbl oncogene reveals an ability for nuclear transport, DNA binding and acute transformation. EMBO J. 12: 2017
3. Joazeiro, C. A., et al. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286: 309
4. Galisteo, M. L., et al. Tyrosine phosphorylation of the c-cbl proto-oncogene protein product and association with epidermal growth factor (EGF) receptor upon EGF stimulation. J. Biol. Chem. 270: 20242-5 (1995).
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