CD22 antibody 
RabMAb®
Cat.#: 1638-1
Rabbit Monoclonal Antibody
Swiss Prot: P20273
Mol Weight: 150kDa
Price: $220
Availability: Ship next business day
Description
CD22, a negative regulator of B cell signaling, belongs to the sialoadhesin family of receptors that preferentially binds to α2-6-linked sialic acid on glycoproteins (1-2). CD22 is known to interact with the B cell antigen receptor (BCR) (3) and the tyrosine phosphatase SHP1. Tyrosine phosphorylation of CD22 leads to the recruitment of multiple intracellular Src homology 2 (SH2) domain-containing effector molecules, including phospholipase C and Syk, which are important for triggering Ca2+ influx, as well as PI 3-kinase and Grb2 (4-5).
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:1,000
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on Raji cell lysate using anti-CD22 RabMAb (cat. #1638-1), 1: 1000 dilution. |
Specificity
A synthetic peptide corresponding to residues near the N-term of human CD22 was used as immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CD22 antibody, FLJ22814 antibody, MGC130020 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, B-cell receptor CD22 antibody, T-cell surface antigen Leu-14 antibody
Description References
1. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H.: J. Immun. 150: 5013-5024, 1993.
2. Nitschke, L., H. Floyd, P. R. Crocker. 2001. Scand. J. Immunol. 53:227
3. Tedder, T. F., J. Tuscano, S. Sato, J. H. Kehrl. 1997. Annu. Rev. Immunol. 15:481
4. Yohannan, J., J. Wienands, K. M. Coggeshall, L. B. Justement. 1999. J. Biol. Chem. 274:18769
5. Otipoby, K. L., K. E. Draves, E. A. Clark. 2001. J. Biol. Chem. 276:44315.
Product References
1. Bannister D, Popovic B, Sridharan S, et al, Epitope mapping and key amino acid identification of anti-CD22 immunotoxin CAT-8015 using hybrid β-lactamase display ,Protein Eng Des Sel 24 (2011) 351-360
2. Cariappa A, Takematsu H, Liu H, et al, B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9- O -acetyl sialic acid esterase ,J. Exp. Med. 206 (2009) 125 - 138
[Application: IP]
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