CD22 Phospho (pY822) antibody 
RabMAb®
Cat.#: 1610-1
Rabbit Monoclonal Antibody
Swiss Prot: P20273
Mol Weight: 150kDa
Price: $285
Availability: Ship next business day
Description
CD22, a negative regulator of B cell signaling, belongs to the sialoadhesin family of receptors that preferentially binds to α2-6-linked sialic acid on glycoproteins (1-2). CD22 is known to interact with the B cell antigen receptor (BCR) (3) and the tyrosine phosphatase SHP1. Tyrosine phosphorylation of CD22 leads to the recruitment of multiple intracellular Src homology 2 (SH2) domain-containing effector molecules, including phospholipase C and Syk, which are important for triggering Ca2+ influx, as well as PI 3-kinase and Grb2 (4-5). Phosphorylation of Tyrosine 822 is involved in the binding of Syk.
Recommended Applications
WB, FC
Applications and Recommended Dilution Factors
WB: 1:1,000
FC: 1:10
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on Raji cell lysate using anti-Phospho-CD22 (pY822) RabMAb (cat. #1610-1), 1:1,000 dilution. Cells were either (A) untreated (B) treated with Pervanadate | B. Flow cytometric analysis of permeabilized K562 cells, untreated (green) or Pervanadate-treated (red) using anti-CD22 (pY822) RabMAb (cat. # 1610-1). |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 822 of human CD22 was used as immunogen. The antibody will detect CD22 phosphorylation on Tyrosine 822. This antibody is predicted to detect splice isoform 2 based on sequence homology.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CD22 antibody, FLJ22814 antibody, MGC130020 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, B-cell receptor CD22 antibody, T-cell surface antigen Leu-14 antibody
Description References
1. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H.: J. Immun. 150: 5013-5024, 1993.
2. Nitschke, L., H. Floyd, P. R. Crocker. 2001. Scand. J. Immunol. 53:227
3. Tedder, T. F., J. Tuscano, S. Sato, J. H. Kehrl. 1997. Annu. Rev. Immunol. 15:481
4. Yohannan, J., J. Wienands, K. M. Coggeshall, L. B. Justement. 1999. J. Biol. Chem. 274:18769
5. Otipoby, K. L., K. E. Draves, E. A. Clark. 2001. J. Biol. Chem. 276:44315.
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