Btk Phospho (pY223) (BTK) antibody 
RabMAb®
Cat.#: 2207-1
Rabbit Monoclonal Antibody
Swiss Prot: Q06187
Mol Weight: 77kDa
Price: $285
Availability: Ship next business day
Description
Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3). Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4).
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
IP: 1:40
Species Reactivity *
Human, Mouse, Rat
*Mouse and Rat reactivity predicted based on sequence alignment only, not based on testing
Products Data
 |
| A. Western blot analysis on Ramos cell lysates using anti-Phospho-BTK (pY223) RabMAb (cat. #2207-1). Cells were either (A) untreated (B) treated with pervanadate. |
Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
BTK antibody, AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, MGC126261 antibody, MGC126262 antibody, PSCTK1 antibody, XLA antibody, Tyrosine-protein kinase BTK antibody, Bruton tyrosine kinase antibody
Description References
1. Tsukada, S., et al (1993) Cell 72, 279-290
2. Rawlings, DJ et al. Immunol. 7, 237-246
3. Vassilev, AZ et al.. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, et al. Mol Cell Biol. 1995;15:5304
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