Alpha-Actinin 1 (ACTN1) antibody RabMAb®

Cat.#: 2279-1
Abcam ID: ab68194

Rabbit Monoclonal Antibody

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Description

Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).

Recommended Applications

WB, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1,000-10,000
IP: 1:10-100
FC: 1:10-100

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on (A) NIH 3T3 (B) HeLa (C) C6 lysates using anti-alpha-actinin 1 RabMAb (cat. # 2279-1), dilution 1:10,000.

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Specificity

A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 1 was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ACTN1 antibody, Alpha-actinin-1 antibody, Alpha-actinin cytoskeletal isoform antibody, F-actin cross-linking protein antibody, Non-muscle alpha-actinin-1 antibody

Description References

1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Otey, CA and Carpen O. Cell Motil. Cytoskeleton 58:104-111, (2004).

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