ATF-2 (C-term) (ATF2) antibody 
RabMAb®
Cat.#: 1489-1
Rabbit Monoclonal Antibody
Swiss Prot: P15336
Mol Weight: 70kDa
Price: $260
Availability: Ship next business day
Description
ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250-500
ICC: 1:250-500
IP: 1:60
Species Reactivity *
Human
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |
| A. Western blot analysis on HeLa cell lysate using anti-ATF-2 (C-term) RabMAb (cat. #1489-1); dilution 1:10,000. | B. Immunohistochemical analysis of paraffin-embedded breast carcinoma using anti-ATF2 (C-term) RabMAb (cat. #1489-1). |
Specificity
A synthetic peptide corresponding to residues in C-terminus of human ATF2 was used as immunogen. The antibody does not cross-react with other ATF family members .
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ATF2 antibody, CRE-BP1 antibody, CREB2 antibody, HB16 antibody, MGC111558 antibody, TREB7 antibody, Cyclic AMP-dependent transcription factor ATF-2 antibody, HB16 antibody, cAMP response element-binding protein CRE-BP1 antibody
Description References
1. Maekawa, T., et al. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J. 8: 2023
2. Gupta, S., et al. Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267: 389
3. van Dam, H., et al. ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J. 14: 1798
4. Livingstone, C., et al. ATF-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14: 1785
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