Btk Phospho (pY551) (BTK) antibody 
RabMAb®
Cat.#: 1685-1
Rabbit Monoclonal Antibody
Swiss Prot: Q06187
Mol Weight: 74kDa
Price: $285*
Availability: Ship next business day
Description
Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). BTK plays a crucial role in B cell activation and development (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3). Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4). Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA). Patients with this disease are unusually prone to bacterial infection but not to viral infection (5).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100-250
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis of anti-Btk phospho (pY551) RabMAb (cat. #1685-1), 1:2,000 dilution A: K562 cell lysate non-treated B: K562 cell lysate + Pervanadate C: Jurkat cell lysate non-treated D: Jurkat cell lysate + Pervanadate |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 551 of human Btk was used as immunogen. The antibody will detect Btk phosphorylation on Tyrosine 551.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
BTK antibody, AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, MGC126261 antibody, MGC126262 antibody, PSCTK1 antibody, XLA antibody, Tyrosine-protein kinase BTK antibody, Bruton tyrosine kinase antibody
Description References
1. Tsukada, S., Saffaran, D., Rawlings, D. & Witte, O. (1993) Cell 72, 279-290
2. Rawlings, D. J. & Witte, O. N. (1995) Semin. Immunol. 7, 237-246
3. Vassilev, A., Z. Ozer, C. Navara, S. Mahajan, and F. M. Uckun. 1999. J. Biol. Chem. 274:1646-1656
4. Mahajan S, Fargnoli J, Burkhardt A L, Kut S A, Saouaf S J, Bolen J B. Mol Cell Biol. 1995;15:5304
5. Yun Qiu, Dan Robinson, Tom G. Pretlow, and Hsing-Jien Kung, Cell Biology, Vol. 95, Issue 7, 3644-3649, March 31, 1998.
Product References
1. Grasis JA, Guimond DM, Cam NR, et al, In Vivo Significance of ITK-SLP-76 Interaction in Cytokine Production ,Mol Cell Biol 30 (2010) 3596-3609
2. Daniel JL, Dangelmaier CA, Mada S, et al, Cbl-b Is a Novel Physiologic Regulator of Glycoprotein VI-dependent Platelet Activation ,J Biol Chem 285 (2010) 17282-17291
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