ACAT (CES1) antibody RabMAb®

Cat.#: 2312-1
Abcam ID: ab68190

Rabbit Monoclonal Antibody

---

Download datasheet [PDF]|Similar Products|Related Products

Bulk and Custom formulation available Email request

---

Description

Triacylglycerol hydrolase is a microsomal enzyme that hydrolyzes stored cytoplasmic triacylglycerol in the liver and participates in the lipolysis/re-esterification cycle during the assembly of very-low-density lipoproteins. Expression of human TGH in Escherichia coli yields a protein without enzymatic activity, which suggests that posttranslational processing is necessary for the catalytic activity (1). ACAT esterifies free cholesterol and stores cholesteryl esters in lipid droplets. Macrophage ACAT1 deficiency results in increased atherosclerotic lesion area in hyperlipidemic mice via disrupted cholesterol efflux, increased lipoprotein uptake, accumulation of intracellular vesicles, and accelerated apoptosis (2). Data show that macrophage ACAT1 influences the efflux of both cellular and lipoprotein-derived cholesterol and propose a pathway for the pro-atherogenic transformation of ACAT1(-/-) macrophages (3).

Recommended Applications

WB, IP, FC

Applications and Recommended Dilution Factors

WB: 1:25,000
IP: 1:30
FC: 1:100

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

---

Products Data

A. Western blot analysis on (A) U937 (B) human small intestine (C) human liver lysate using anti-ACAT RabMAb (cat. # 2312-1), dilution 1:50,000.

---

Specificity

A synthetic peptide corresponding to residues on the C-terminus of human ACAT was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

CES1 antibody, ACAT antibody, CE-1 antibody, CEH antibody, CES2 antibody, HMSE antibody, HMSE1 antibody, PCE-1 antibody, REH antibody, SES1 antibody, TGH antibody, hCE-1 antibody, Liver carboxylesterase 1 antibody, Serine esterase 1 antibody, Triacylglycerol hydrolase antibody

Description References

1. Alam M, et al. Biochemistry 41(21):6679-87, 2002.
2. Dove DE, et al. Atherosclerosis 186(2):267-74, 2006.
3. Sone DE, et al. Arterioscler Thromb Vasc Biol 25(1): 128-34, 2005.

Similar Products

Product Name	Catalog #
ACAT (C-term) RabMAb®	1912-1
ACAT	1864-1

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG (H+L) HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1