WASP antibody RabMAb®

Cat.#: 2304-1
Abcam ID: ab68182

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

The Wiskott-Aldrich syndrome (WAS) is an X-chromosome-linked recessive disease characterized by eczema, thrombocytopenia, and immunodeficiency (1). WAS is an immunodeficiency disorder with the most severe pathology in the T lymphocytes and platelets. The disease arises from mutations in the gene encoding the WAS protein (WASP). T lymphocytes of affected males with WAS exhibit a severe disturbance of the actin cytoskeleton, suggesting that the WASP could regulate its organization. Data suggest that the WASP might function as a signal transduction adaptor downstream of Cdc42, and in affected males, the cytoskeletal abnormalities may result from a defect in Cdc42 signaling (2). WASP is a key regulator of the Arp2/3 complex and the actin cytoskeleton in hematopoietic cells. WASP is capable of forming an auto-inhibited conformation, which can be disrupted by binding of Cdc42 and phosphatidylinositol 4,5-bisphosphate, leading to its activation. Stimulation of the collagen receptor on platelets and crosslinking the B-cell receptor induce tyrosine phosphorylation of WASP (3).

Recommended Applications

WB, ICC, IP, FC

Applications and Recommended Dilution Factors

WB: 1:1000 - 2000
ICC: 1:100 - 250
IP: 1:30
FC: 1:30

Species Reactivity *

Human, Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on (A) TF-1 (B) U937 cell lysate and (C) Jurkat cell lysate using anti-WASP RabMAb (cat. # 2304-1), dilution 1:2000.

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Specificity

A synthetic peptide corresponding to residues near the N-terminus of human WASP was used as an immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

WAS antibody, IMD2 antibody, SCNX antibody, THC antibody, THC1 antibody, WASP antibody, Wiskott-Aldrich syndrome protein antibody

Description References

1. Smith FJ, et al. Biochem Biophys Res Commun. 297(4):818-27, 2002.
2. Adley BP, et al. Anal Quant Cytol Histol 28(4):228-36, 2006
3. Coons SW, et al. Endocr Pathol 16(3):201-10, 2005

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