Phospho-p53-T18 Antibody
Cat.#: T3766Rabbit Polyclonal Antibody
Swiss Prot: P04637
Mol Weight: 44kDa
Price: $289*
Availability: Ship 4-5 business days
Description
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Recommended Applications
WB, IHC, ELISA
Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
ELISA: 1:1,000
Species Reactivity
Human, Mouse
Products Data
 |  |  |
| Dot blot analysis of anti-hp53-T18 Phospho-specific Pab on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml. | Western blot analysis of anti-Phospho-p53-T18 Pab in, from left to right, A2058, Ramos, mouse lung, mouse testis, and HL60 cell line lysates. Phospho-p53-T18(arrow) was detected using the purified Pab. | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Specificity
This Phospho-p53-T18 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
Garden, G.A., et al., Biochem. Biophys. Res. Commun. 331(3):799-809 (2005).
Castedo, M., et al., Biochem. Biophys. Res. Commun. 331(3):701-706 (2005).
Duensing, A., et al., Biochem. Biophys. Res. Commun. 331(3):694-700 (2005).
Seemann, S., et al., Oncogene 24(24):3853-3863 (2005).
Mann, K., et al., Oncogene 24(24):3964-3975 (2005).
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