Phospho-p27Kip1-T157 Antibody
Cat.#: T3757Rabbit Polyclonal Antibody
Swiss Prot: P46527
Mol Weight: 22kDa
Price: $289*
Availability: Ship 4-5 business days
Description
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Recommended Applications
WB, IHC, ELISA
Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
ELISA: 1:1,000
Species Reactivity
Human
Products Data
 |  |
| The anti-Phospho-p27Kip1-T157 Pab is used in Western blot to detect Phospho-p27Kip1-T157 in HL60 tissue lysate | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Specificity
This Phospho-p27Kip1-T157 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Names
CDKN1B antibody, CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, Cyclin-dependent kinase inhibitor 1B antibody, Cyclin-dependent kinase inhibitor p27 antibody, p27Kip1 antibody
Description References
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005).
Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005).
Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005).
Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005).
Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
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