Phospho-GSK3B-S21/29 Antibody
Cat.#: T3720Rabbit Polyclonal Antibody
Swiss Prot: P49841
Mol Weight: 47kDa
Price: $289*
Availability: Ship 4-5 business days
Description
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.
Recommended Applications
WB, IHC, ELISA
Applications and Recommended Dilution Factors
WB: 1:250~500
IHC: 1:50~100
ELISA: 1:1,000
Species Reactivity
Human, Mouse
Products Data
 |  |
| The anti-Phospho-GSK3B-S21/29 Pab is used in Western blot to detect Phospho-GSK3B-S21/29 in mouse thymus tissue lysate. | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Specificity
This Phospho-GSK3B-S21/29 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 6~36 amino acids surrounding S21 and S29 of human GSK3B.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Names
GSK3B antibody, - antibody, Glycogen synthase kinase-3 beta antibody, antibody
Description References
Wang, L., et al., J. Biol. Chem. 279(31):32444-32452 (2004).
Yuan, Z., et al., J. Biol. Chem. 279(25):26105-26114 (2004).
Liao, X., et al., Endocrinology 145(6):2941-2949 (2004).
Salas, T.R., et al., J. Biol. Chem. 279(18):19191-19200 (2004).
Takahashi-Yanaga, F., et al., Biochem. Biophys. Res. Commun. 316(2):411-415 (2004).
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