Phospho-CHK1-S280 Antibody
Cat.#: T3702Rabbit Polyclonal Antibody
Swiss Prot: O14757
Mol Weight: 54kDa
Price: $289*
Availability: Ship 4-5 business days
Description
Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Recommended Applications
WB, IHC, ELISA
Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
ELISA: 1:1,000
Species Reactivity
Human, Mouse
Products Data
 |
| The anti-Phospho-CHK1-S280 Pab is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, mouse brain, Ramos, Jurkat, HL60, and Hela tissue lysates. |
Specificity
This Phospho-CHK1-S280 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Names
CHEK1 antibody, CHK1 antibody, Serine/threonine-protein kinase Chk1 antibody, antibody
Description References
Kramer, A., et al., Nat. Cell Biol. 6(9):884-891 (2004).
Xu, X., et al., J. Biol. Chem. 279(33):34091-34094 (2004).
Ng, C.P., et al., J. Biol. Chem. 279(10):8808-8819 (2004).
Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003).
Groth, A., et al., EMBO J. 22(7):1676-1687 (2003).
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