Phospho-Bad-S99 Antibody
Cat.#: T3693Rabbit Polyclonal Antibody
Swiss Prot: Q92934
Mol Weight: 18kDa
Price: $289*
Availability: Ship 4-5 business days
Description
Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
Recommended Applications
WB, IHC, ELISA
Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
ELISA: 1:1,000
Species Reactivity
Human
Products Data
 |  |
| Dot blot analysis of anti-hBad-pS99 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Specificity
This Phospho-Bad-S99 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Names
BAD antibody, BBC2 antibody, BCL2L8 antibody, Bcl2 antagonist of cell death antibody, Bcl-XL/Bcl-2-associated death promoter antibody
Description References
Hurbin, A., et al., J. Biol. Chem. 280(20):19757-19767 (2005).
Antignani, A., et al., Biochemistry 44(10):4074-4082 (2005).
Ying, S., et al., Infect. Immun. 73(3):1399-1403 (2005).
Seo, S.Y., et al., J. Biol. Chem. 279(40):42240-42249 (2004).
Lee, J.W., et al., Carcinogenesis 25(8):1371-1376 (2004).
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