We're sorry this product is no longer available.
Phospho-H3-S28 AntibodyCat.#: T3679
Rabbit Polyclonal Antibody
Swiss Prot: P68431
Mol Weight: 15kDa
Availability: Ship 4-5 business days
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Applications and Recommended Dilution Factors
|Western blot analysis of anti-Phospho-H3-pS28 Pab in HepG2 cell line lysate (35ug/lane). Mouse Phospho-H3-pS28(arrow) was detected using the purified Pab (1:64 dilution).|
This Phospho-H3-S28 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S28 of human H3.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
HIST1H3J antibody, H3/j antibody, H3FJ antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody
Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003).
Deng, L., et al., Virology 289(2):312-326 (2001).
Deng, L., et al., Virology 277(2):278-295 (2000).
El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998).
Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).
|Product Name||Catalog #|
|Goat Anti-Rabbit IgG (H+L) HRP||3053-1|
|Goat Anti-Rabbit IgG polymerized HRP||3051-1|
|Cell Lysis Buffer||3500-1|
|Goat Anti-Rabbit IgG HRP Antibody||3056-1|