p53 Phospho (pS392) (TP53) antibody 
RabMAb®
Cat.#: 2326-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285*
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair. Various serine/threonine kinases (ATM, p38, Chk) has been implicated to p53 phosphorylation. Phosphorylation at serine 392 has been linked to p53 activation. Phosphorylation on serine 392 also induces p53 interaction with NF kappa beta - p65 in mouse and inhibition with Cdc2 and MDM2 in human (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. p53 is frequently mutated or inactivated in about 60% of cancers.
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:5000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:40
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |  |
| A.Western blot analysis on HepG2 cell lysate using anti-phospho-p53 (pS392) RabMAb (cat. #2326-1), 1:10,000 dilution. Cells were either (A) untreated or (B) treated with etoposide. | B. Immunohistochemical analysis of paraffin-embedded human ovarian adenocarcinoma using anti-Phospho-p53 RabMAb (cat# 2326-1) | C. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-Phospho-p53 RabMAb (cat# 2326-1) |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Serine 392 of human p53 was used as an immunogen. The antibody will detect p53 phosphorylation on Serine 392.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Thompson T, et al. JBC 279:53015-53022, 2004.
2. Jeong SJ, et al. Blood 104:1490-1497, 2004
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