PDGF Receptor beta Phospho (pY740) (PDGFRB) antibody RabMAb®

Cat.#: 2213-1
Abcam ID: ab81301

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Human platelet-derived growth factor (PDGF) receptor is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others. Both Tyrosine 751 and 740 is required for PDGFR-β mediated PI3 kinase activation. In particular, Tyrosine 751 is the docking site for P13 kinase (4).

Recommended Applications

WB, ICC

Applications and Recommended Dilution Factors

WB: 1:1000 - 10,000
ICC: 1:100

Species Reactivity *

Mouse, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on NIH 3T3 cell lysates using anti-Phospho-PDGF receptor beta (pY740) RabMAb (cat. #2213-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with PDGF

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Specificity

A phospho specific peptide corresponding to residues surrounding Tyrosine 740 of human PDGF-Receptor β was used as an immunogen. This antibody detects PDGF-Receptor β phosphorylated at Tyrosine 740

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

PDGFRB antibody, CD140B antibody, IBGC4 antibody, JTK12 antibody, PDGFR antibody, PDGFR-1 antibody, PDGFR1 antibody, Platelet-derived growth factor receptor beta antibody, Beta platelet-derived growth factor receptor antibody, Beta-type platelet-derived growth factor receptor antibody, CD140 antigen-like family member B antibody, Platelet-derived growth factor receptor 1 antibody

Description References

1. Deuel, T.F., et al. Biofactors 1: 213
2. Deuel, T.F., et al. J Clin Invest. 74: 669
3. Kazlauskas, A, et al. Cell 58: 1121
4. Panayotou, G. et al. EMBO J. 11:4261-4272, 1992.

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