FAK Phospho (pY397) (PTK2) antibody RabMAb®

Cat.#: 2211-1
Abcam ID: ab81298

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation.

Recommended Applications

WB

Applications and Recommended Dilution Factors

WB: 1:1000-2000

Species Reactivity *

Human, Rat

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on human brain tissue lysates (A, B) and rat brain tissue lysates (C, D) using anti-Phospho-Fak (pY397) RabMAb (cat. #2211-1), 1:2000 dilution. Cells were either (A, C) untreated (B, D) treated with AP

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Specificity

A phospho-specific peptide corresponding to residues surrounding Tyrosine 397 of human FAK was used as an immunogen. This antibody detects FAK phosphorylated at Y397.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

PTK2 antibody, FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, Focal adhesion kinase 1 antibody, Protein-tyrosine kinase 2 antibody, p125FAK antibody, pp125FAK antibody

Description References

1. Schaller, M.D. Biochim. Biophys. Acta 1540, 1
2. Schaller, M.D., et al; .PNAS. 89:5192
3. Salazar, E.P, et al. J. Biol. Chem. 274:28371
4. Tremblay L., et al. Int. J. Cancer, 68: 164-171, 1996

Product References

1. Barnes EA, Kenerson HL, Jiang X, et al, Tuberin Regulates E-Cadherin Localization: Implications in Epithelial-Mesenchymal Transition ,Am J Pathol 177 (2010) 1765-1778

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