ZAP-70 Phospho (pY492) (ZAP70) antibody 
RabMAb®
Cat.#: 2215-1
Rabbit Monoclonal Antibody
Swiss Prot: P43403
Mol Weight: 70kDa
Price: $285*
Availability: Ship next business day
Description
Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Lck phosphorylates Tyrosine 492 and 493 located at the activation loop of the catalytic domain of ZAP-70, leading to its activation and increased autophosphorylation (3).
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IP: 1:30
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on Jurkat cell lysates using anti-Phospho-ZAP-70 (p492) RabMAb (cat. #2215-1). Cells were either (A) untreated (B) treated with pervanadate |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Tyr492 of human ZAP-70 was used as immunogen. The antibody only detects ZAP-70 phosphorylated on Tyrosine 492.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ZAP70 antibody, FLJ17670 antibody, FLJ17679 antibody, SRK antibody, STD antibody, TZK antibody, ZAP-70 antibody, Tyrosine-protein kinase ZAP-70 antibody, 70 kDa zeta-associated protein antibody, Syk-related tyrosine kinase antibody
Description References
1. Chu, D.H., et al. Immunol. Rev. 165:167
2. Isakov, N., et al. J Exp Med. 181:375
3. Isakov, N., et al. J Exp Med. 181:375
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