We're sorry this product is no longer available.
4E-BP1 Phospho (pS65) (EIF4EBP1) antibody 
RabMAb®
Cat.#: 2206-1
Rabbit Monoclonal Antibody
Swiss Prot: Q13541
Mol Weight: 20kDa
Price: $285*
Availability: Call for availability
Description
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 65. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:2000
Species Reactivity *
Human, Mouse
*Cross reactivity determined by western blot only.
Products Data
 |
| A. Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-4E-BP1 (pS65) (EIF4EBP1) RabMAb (cat. #2206-1). Cells were either (A) untreated (B) treated with PDGF. |
Specificity
A phospho-specific peptide corresponding to residues surrounding serine 65 of human 4E-BP1 was used as an immunogen. This antibody detects 4E-BP1 phosphorylated at serine 65.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
EIF4EBP1 antibody, 4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, MGC4316 antibody, PHAS-I antibody, Eukaryotic translation initiation factor 4E-binding protein 1 antibody, Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Description References
1. Pause, A., 1994. Nature 371: 762-767
2. Gingras, A.-C. 1998. Genes & Dev. 12: 502-513
3. Iritani, B. M, (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180
4. Trumpp, A., (2001) Nature 414, 768
Similar Products
| Product Name | Catalog # |
|---|---|
| 4E-BP1 RabMAb® | 1578-1 |
| 4E-BP1 RabMAb® | 1557-1 |
| 4E-BP1 Phospho (pT70) RabMAb® | 2250-1 |
| 4E-BP1/2/3 Phospho (pT45) RabMAb® | 2334-1 |
| 4E-BP1 Phospho (pT37) RabMAb® | 2411-1 |
Related Products
| Product Name | Catalog # |
|---|---|
| Goat Anti-Rabbit IgG (H+L) HRP | 3053-1 |
| Goat Anti-Rabbit IgG polymerized HRP | 3051-1 |
| Cell Lysis Buffer | 3500-1 |
| Goat Anti-Rabbit IgG HRP Antibody | 3056-1 |
| Goat Anti-Rabbit IgG DyLight® 405 | 3063-1 |
| Goat Anti-Rabbit IgG DyLight® 488 | 3064-1 |
| Goat Anti-Rabbit IgG DyLight® 550 | 3065-1 |
| Goat Anti-Rabbit IgG DyLight® 594 | 3066-1 |
| Goat Anti-Rabbit IgG DyLight® 633 | 3067-1 |
| Goat Anti-Rabbit IgG DyLight® 650 | 3068-1 |
