p53 Phospho (pS46) (TP53) antibody 
RabMAb®
Cat.#: 2190-1
Rabbit Monoclonal Antibody
Swiss Prot: P04637
Mol Weight: 53kDa
Price: $285*
Availability: Ship next business day
Description
p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers. Due to UV-induced DNA damage, p53 is phosphorylated on eight different serine/threonine locations on the N-terminal. Phosphorylation on serine 46 has been implicated as a mediator in apoptosis induced by DNA-damage (4, 5).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1000 - 10,000
IHC: 1:100 - 250
ICC: 1:50
IP: 1:40
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
 |  |
| A. Western blot analysis on HepG2 cell lysates using anti-p53 Phospho (pS46) RabMAb (cat. #2190-1), 1:2000 dilution. Cells were either (A) untreated (B) treated with etoposide. | B. Immunohistochemical analysis of human ovarian adenocarcinoma using anti-p53 Phospho (pS46) RabMAb (Cat# 2190-1) |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding serine 46 of human p53 protein.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
TP53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, Cellular tumor antigen p53 antibody, Antigen NY-CO-13 antibody, Phosphoprotein p53 antibody, Tumor suppressor p53 antibody
Description References
1. Levine, A.J.; Cell 88: 323
2. Lakin, N.D. S.P. Jackson. Oncogene 18: 7644-7655,1999
3. Vogelstein, B.; Nature 348: 681
4. Hoolstein, M., et al. Science 253: 49
5. Saito, S., et al. J. Biol. Chem. 277:12491
Product References
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