p27/Kip1 Phospho (pS10) (CDKN1B) antibody 
RabMAb®
Cat.#: 2187-1
Rabbit Monoclonal Antibody
Swiss Prot: P46527
Mol Weight: 27kDa
Price: $285*
Availability: Ship next business day
Description
p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
Recommended Applications
WB, IHC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100
IP: 1:30
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |  |
| A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (cat. #2187-1), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP. | B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Phospho-p27Kip1 RabMAb (cat. #2187-1) | C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-p27Kip1 RabMAb. (cat. # 2187-1). |
Specificity
A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
CDKN1B antibody, CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, Cyclin-dependent kinase inhibitor 1B antibody, Cyclin-dependent kinase inhibitor p27 antibody, p27Kip1 antibody
Description References
1. Polyak K., et al; Cell 78:59-66,1994.
2. Carrano,A.C., et al. Nat. Cell Biol., 1, 193-199
3. Rodier,G.,et al. EMBO J., 20, 6672-6682, 2001
Product References
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