Histone H3 (pT3) Phospho (HIST1H3J) antibody RabMAb®

Cat.#: 2162-1
Abcam ID: ab78351

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr 3 in prophase and its dephosphorylation during anaphase. Furthermore we find that haspin, a member of a distinctive group of protein kinases present in diverse eukaryotes, phosphorylates H3 at Thr 3 in vitro. (5).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity *

Human

*Cross reactivity determined by western blot only.

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Products Data

A. Western blot analysis on HeLa cell lysates using anti-Phospho-Histone H3 (pT3) RabMAb (cat. #2162-1). Cells were either (A) untreated (B) treated with FBS + Calyculin A.	B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Histone H3 RabMAb (cat. # 2162-1).

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Specificity

A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

HIST1H3J antibody, H3/j antibody, H3FJ antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

Description References

1. Braunstein, M., et al. Mol. Cell. Biol. 16: 4349
2. Workman, J.L. and R.E. Kingston. Annu. Rev. Biochem. 67: 545
3. Hansen, J.C. et al. Biochemistry 37, 17637
4. Strahl, B.D. and C.D. Allis. Nature 403, 41
5. Dai J et al. Genes Dev 19(4):472-88, 2005

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