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Tandem IHC Detection Kit I antibody RabMAb®

Cat.#: 4000-1

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• Dual color IHC detection
• Pair Epitomics RabMAbs with any mouse mAb
• Optimized for use with Paraffin embedded tissues
• Strong specific staining with clean background
• Complete ready to use kits including detection reagents

Description

Double staining is a technique used to evaluate the expression of two distinct antigens in the same tissue sample. The advent of Rabbit Monoclonal antibodies (RabMAbs) has opened up the possibility of performing reliable and clean dual IHC staining of formalin fixed, paraffin-embedded tissue samples. Until now, the majority of staining protocols involved biotinylated primary antibodies or using a combination of monoclonal and polyclonal antibodies. These procedures generally provide inconsistent staining with high background. RabMAbs can be easily paired with mouse monoclonal antibodies in a straightforward procedure that results in beautiful, clean dual labeled samples. Tandem IHC Kits are offered without our primary rabbit monoclonal. The Detection Kits contain the detection reagents only enabling researchers to pair any suitable Epitomics rabbit monoclonal antibody with any mouse monoclonal of their choice. Each Tandem IHC Kits is supplied with sufficient reagents to perform up to 50 stainings.

Recommended Applications

IHC

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Products Data

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Components

• Antigen Retrieval solution (1X)
• Peroxidase Block solution
• Blocking solution
• Primary antibody diluent
• Negative control rabbit IgG (1X)
• Secondary anti-Rabbit (HRP)
• Secondary anti-Mouse (AP)
• DAB A
• DAB B
• Fast Red tablets
• Fast Red solution

Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

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