Beta-Catenin (CTNNB1) antibody RabMAb®

Cat.#: 1247-1
Abcam ID: ab32572

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

β-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation (1). Beta-catenin is phosphorylated at four serine and threonine residues at the N-terminus (2). Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33 (2). Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation (3). Mutations in the Beta-catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas (4).

Recommended Applications

WB, IHC, ICC, IP

Applications and Recommended Dilution Factors

WB: 1:5,000-10,000
IHC: 1:500
ICC: 1:250
IP: 1:100

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on A431 cell lysate using anti-Beta-catenin RabMAb (cat. #1247-1); dilution 1:10,000.	B. Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Beta-catenin RabMAb (cat. #1247-1).	C. Immunofluorescent staining of A431 cells using anti-Beta-catenin RabMAb (cat. #1247-1).

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Specificity

A synthetic peptide corresponding to residues near N-terminus of human β-catenin was used as immunogen.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

CTNNB1 antibody, CTNNB antibody, Catenin beta-1 antibody, Beta-catenin antibody

Description References

1. Kikuchi, A. Regulation of beta-catenin signaling in the Wnt pathway. Biochem Biophys Res Commun. 268: 243
2. Hagen, T., and A. Vidal-Puig. Characterisation of the phosphorylation of beta-catenin at the GSK-3 priming site Ser45. Biochem Biophys Res Commun. 294: 324
3. van Noort, M., et al. Wnt signaling controls the phosphorylation status of beta-catenin. J Biol Chem. 277: 17901
4. Blaker, H., et al. Beta-catenin accumulation and mutation of the CTNNB1 gene in hepatoblastoma. Genes Chromosomes Cancer. 25: 399

Product References

1. Dekkena HV, Winka JC, Vissersa KJ, et al, Wnt pathway-related gene expression during malignant progression in ulcerative colitis ,Acta histochemica 109 (2007) 266-272
[Application: IHC]
2. Li X, Gonzalez ME, Toy K, et al, Targeted Overexpression of EZH2 in the Mammary Gland Disrupts Ductal Morphogenesis and Causes Epithelial Hyperplasia ,Am. J. Pathol. 175 (2009) 1246 - 1254
3. Gaspar C, Franken P, Molenaar L, et al, A Targeted Constitutive Mutation in the Apc Tumor Suppressor Gene Underlies Mammary But Not Intestinal Tumorigenesis ,PLoS Genet 5 (2009) e1000547
[Application: IHC]

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