Histone H3 Acetyl (K56) (HIST1H3J) antibody RabMAb®

Cat.#: 2134-1
Abcam ID: ab76307

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4).

Recommended Applications

WB, IHC, ICC

Applications and Recommended Dilution Factors

WB: 1:1000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250

Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on C6 cell lysate using anti-Histone H3 Acetyl-K56 RabMAb (cat. #2134-1). Cells were either (A) untreated or (B) treated with TSA.	B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-Histone 3 RabMAb (cat. #2134-1)	C. Immunofluorescent staining of HeLa cells using anti-Histone 3 RabMAb (cat. #2134-1)

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Specificity

A synthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 56.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

HIST1H3J antibody, H3/j antibody, H3FJ antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

Description References

1. Braunstein M, et al. Mol. Cell. Biol. 16:4349
2. Workman JL, Kingston RE, Annu. Rev. Biochem. 67: 545
3. Cheung, P. et al. Cell103, 263
4. Xiao T, et al. Mol Cell Bio 25:637-651, 2005
5. Ahn SH, et al. Cell Cycle 4:780-783, 2005

Product References

1. Xie W, Song CY, Young NL, et al, Histone H3 Lysine 56 Acetylation Is Linked to the Core Transcriptional Network in Human Embryonic Stem Cells ,Molecular Cell 33 (2009) 417-427
[Application: WB]
2. Wirth M, Paap F, Fischle W, et al, HIS-24 Linker Histone and SIR-2.1 Deacetylase Induce H3K27me3 in the Caenorhabditis elegans Germ Line ,Mol. Cell. Biol. 29 (2009) 3700 - 3709
3. Wirth M, Paap F, Fischle W, et al, Linker histone HIS-24 and SIR-2.1 deacetylase induce H3K27me3 in C. elegans germline ,Mol. Cell. Biol. 29 (2009) 3700-3709
[Application: ICC]
4. Das C, Lucia MS, Hansen KC, et al, CBP/p300-mediated acetylation of histone H3 on lysine 56 ,Nature 459 ( 2009) 113-117
[Application: WB, FC]
5. Kimbrel EA, Lemieux ME, Xia X, et al, Systematic in vivo structure-function analysis of p300 in hematopoiesis ,Blood 114 (2009) 4804 - 4812
6. Tennen RI, Berber E, Chua KF, Functional dissection of SIRT6: Identification of domains that regulate histone deacetylase activity and chromatin localization ,Mech Ageing Dev 131 (2010) 185-192
[Application: WB]
7. Dutta D, Ray S, Home P, et al, Regulation of Angiogenesis by Histone Chaperone HIRA-mediated Incorporation of Lysine 56-acetylated Histone H3.3 at Chromatin Domains of Endothelial Genes ,J Biol Chem 285 (2010) 41567-41577

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