Beta-Arrestin 1 Phospho (pS412) (ARRB1) antibody 
RabMAb®
Cat.#: 1109-1
Rabbit Monoclonal Antibody
Swiss Prot: P49407
Mol Weight: 50kDa
Price: $285*
Availability: Ship next business day
Description
Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). Beta-arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for Beta-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).
Recommended Applications
WB, IHC, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:500
ICC: 1:100-1:250
IP: 1:100
Species Reactivity *
Human, Mouse, Rat
*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
 |  |
| A. Western blot analysis on 293T cell lysate using anti-Phospho-Beta-Arrestin-1 (pS412) RabMAb (cat. #1109-1); dilution 1:20,000. | B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Phospho-Beta-Arrestin-1 (pS412) RabMAb (cat. #1109-1). |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human Beta Arrestin-1 was used as immunogen. The antibody only detects Beta Arrestin-1 phosphorylated on Serine 412.
Storage Condition and Buffer
Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.
Alternative Names
ARRB1 antibody, ARB1 antibody, ARR1 antibody, Beta-arrestin-1 antibody, Arrestin beta-1 antibody
Description References
1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677
2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971
3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640
4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139
Product References
1. Mandell JW, Glass G, Gianchandani EP, et al, Dephosphorylation of β-Arrestin 1 in Glioblastomas ,J Neuropathol Exp Neurol. 68 (2009) 535-541
[Application: WB, IHC]
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