Beta-Arrestin 1 (ARRB1) antibody RabMAb®

Cat.#: 1274-1
Abcam ID: ab32099

Rabbit Monoclonal Antibody

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Bulk and Custom formulation available Email request

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Description

Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). -arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for -arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).

Recommended Applications

WB, IHC, ICC, FC

Applications and Recommended Dilution Factors

WB: 1:1000-10,000
IHC: 1:100
ICC: 1:100
FC: 1:40

Species Reactivity *

Human, Mouse, Rat

*For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

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Products Data

A. Western blot analysis on 293 cell lysate using anti-ß-Arrestin-1 RabMAb (cat. #1274-1); dilution 1:500.	B. Immunohistochemical analysis of paraffin-embedded human lymph node using anti-ß-arrestin RabMAb (cat. #1274-1).

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Specificity

A synthetic peptide corresponding to residues near N-terminus of human -arrestin-1 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

ARRB1 antibody, ARB1 antibody, ARR1 antibody, Beta-arrestin-1 antibody, Arrestin beta-1 antibody

Description References

1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677
2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971
3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640
4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139

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