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NUP155 Antibody (N-term)

Cat.#: T1594

Rabbit Polyclonal Antibody

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Description

Nucleoporins are the main components of the nuclear pore complex (NPC) of eukaryotic cells. They are involved in the bidirectional trafficking of molecules, especially mRNAs and proteins, between the nucleus and the cytoplasm. The protein does not contain the typical FG repeat sequences found in most vertebrate nucleoporins.

Recommended Applications

WB, IHC, FC, ELISA

Applications and Recommended Dilution Factors

WB: 1:50~100
IHC: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity

Human

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Products Data

Western blot analysis of NUP155 antibody (N-term) in HL60 cell line lysates (35ug/lane). NUP155 (arrow) was detected using the purified Pab.

(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with NUP155 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using NUP155 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specificity

This NUP155 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16~46 amino acids from the N-terminal region of human NUP155.

Storage Condition and Buffer

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names

NUP155 antibody, N155 antibody, Nuclear pore complex protein Nup155 antibody, 155 kDa nucleoporin antibody, Nucleoporin Nup155 antibody

Description References

Zhang,X., Cell 135 (6), 1017-1027 (2008)
Rayala,H.J., Mol. Cell Proteomics 3 (2), 145-155 (2004)

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