ETHE1 Antibody (C-term)

Cat.#: T1569
Abcam ID: ab135589

Rabbit Polyclonal Antibody

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Description

ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.

Recommended Applications

WB, IHC, FC, ELISA

Applications and Recommended Dilution Factors

WB: 1:50~100
IHC: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity

Human, Mouse

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Products Data

Western blot analysis of ETHE1 Antibody (C-term) in mouse liver tissue lysates (35ug/lane).ETHE1 (arrow) was detected using the purified Pab.

(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ETHE1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. (RIGHT)Flow cytometric analysis of 293 cells using ETHE1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specificity

This ETHE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187~217 amino acids from the C-terminal region of human ETHE1.

Storage Condition and Buffer

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Alternative Names

ETHE1 antibody, HSCO antibody, YF13H12 antibody, Protein ETHE1, mitochondrial antibody, Ethylmalonic encephalopathy protein 1 antibody, Hepatoma subtracted clone one protein antibody

Description References

Tiranti,V., Nat. Med. 15 (2), 200-205 (2009)
Mineri,R., J. Med. Genet. 45 (7), 473-478 (2008)

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