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Phospho-IKKg-S31 AntibodyCat.#: T1168
Rabbit Polyclonal Antibody
Swiss Prot: Q9Y6K9
Mol Weight: 48kDa
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IKKg is a regulatory subunit part of the IKK-signalosome complex activation. It is also considered to be a mediator for TAX activation of NF-kappa-B. Additionally, this protein could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Mutations in IKKg are the cause of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) and of familial incontinentia pigmenti type II (IP2)
WB, IHC, ELISA
Applications and Recommended Dilution Factors
|The anti-Phospho-IKKg-S31 Pab is used in Western blot to detect Phospho-IKKg-S31 in mouse cerebellum tissue lysate||Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.|
This Phospho-IKKg-S31 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S31 of human IKKg.
Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
IKBKG antibody, AMCBX1 antibody, FIP-3 antibody, FIP3 antibody, Fip3p antibody, IKK-gamma antibody, IP antibody, IP1 antibody, IP2 antibody, IPD2 antibody, NEMO antibody, ZC2HC9 antibody, NF-kappa-B essential modulator antibody, NF-kappa-B essential modifier antibody
Reiley, W., et al., Mol. Cell. Biol. 25(10):3886-3895 (2005).
Wilson, G.M., et al., Mol. Biol. Cell 16(2):849-860 (2005).
Nishikomori, R., et al., Blood 103(12):4565-4572 (2004).
Orange, J.S., et al., J. Allergy Clin. Immunol. 114(3):650-656 (2004).
Saito, K., et al., Structure (Camb.) 12(9):1719-1728 (2004).
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